Regulatory

Part:BBa_M36150:Design

Designed by: Monica Chin   Group: Stanford BIOE44 - S11   (2014-10-23)


nifRLA promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected the genomic region upstream of the nifL repressor but following the nifR repressor binding site. This is the general region where the nifRLA promoter is found.


Source

The nifRLA promoter sequence was determined from the nif gene cluster sequence for Azotobacter vinelandii. It is found upstream of the nifL repressor.

References